Actin (muscle specific) monoclonal antibody, clone MSA/953
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of human muscle actin.
Immunogen
A synthetic peptide corresponding to human muscle actins.
Host
Mouse
Reactivity
Human, Rat
Specificity
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium.
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/million cells in 0.1 mL)
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western Blot analysis of hSKM cell lysate with Actin (muscle specific) monoclonal antibody, clone MSA/953 (Cat # MAB14634).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of (A) human rhabdomyosarcoma (B) human leiomyosarcoma (C) rat lung (D) rat heart (E) rat stomach and (F) rat skeletal muscle with Actin (muscle specific) monoclonal antibody, clone MSA/953 (Cat # MAB14634).Immunofluorescence
Flow Cytometry
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Gene Info — ACTA1
Entrez GeneID
58Protein Accession#
P62736; P68032; P68133Gene Name
ACTA1
Gene Alias
ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3
Gene Description
actin, alpha 1, skeletal muscle
Gene Ontology
HyperlinkGene Summary
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq
Other Designations
OTTHUMP00000036123|alpha skeletal muscle actin
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Gene Info — ACTA2
Entrez GeneID
59Protein Accession#
P62736; P68032; P68133Gene Name
ACTA2
Gene Alias
AAT6, ACTSA
Gene Description
actin, alpha 2, smooth muscle, aorta
Omim ID
102620Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000020042|alpha 2 actin|alpha-cardiac actin|growth-inhibiting gene 46
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Gene Info — ACTC1
Entrez GeneID
70Protein Accession#
P62736; P68032; P68133Gene Name
ACTC1
Gene Alias
ACTC, CMD1R, CMH11
Gene Description
actin, alpha, cardiac muscle 1
Gene Ontology
HyperlinkGene Summary
Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq
Other Designations
cardiac muscle alpha actin 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Actin cytoskeleton. Setting the pace of cell movement.
Barkalow K, Hartwig JH.
Current Biology 1995 Sep; 5(9):1000.
Application:IF, IHC, WB-Ce, WB-Ti, WB-Tr, Human, Mouse, Mammalian cells, Tissues.
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Actin cytoskeleton. Setting the pace of cell movement.
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