EPCAM recombinant monoclonal antibody, clone HEA125
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Specification
Product Description
Mouse recombinant monoclonal antibody raised against human EPCAM.
Antibody Species
Mouse
Immunogen
Original antibody is raised against the human colon carcinoma cell line HT-29.
Reactivity
Human
Form
Liquid
Isotype
IgG1 kappa
Recommend Usage
Flow Cytometry
Immunofluorescence
Immunohistochemistry
Immunoprecipitation
Radioimmunoassay
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of Lane 1: human breast carcinoma and Lane 2: human colorectal carcinoma tissue lysate with EPCAM recombinant monoclonal antibody, clone HEA125 (Cat # RAB03429).
Western Blot analysis the tissue lysate (35 ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of RAB03429 at 1 ug/mL before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.Immunohistochemistry
Immunofluorescence
Immunofluorescent staining of Caco-2 cells with EPCAM recombinant monoclonal antibody, clone HEA125 (Cat # RAB03429).
Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells stained with the chimeric mouse IgG version of RAB03429 at 10 ug/mL followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with anti-unknown antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03429
(B) DAPI
(C) Merged channels
(D) Isotype controlImmunoprecipitation
Radioimmunoassay
Flow Cytometry
Flow cytometric analysis of Caco-2 cells with EPCAM recombinant monoclonal antibody, clone HEA125 (Cat # RAB03429).
Caco-2 cells were stained with anti-unknown IgG antibody (isotype control-black line) or the mouse IgG chimeric version of RAB03429 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — EPCAM
Entrez GeneID
4072Gene Name
EPCAM
Gene Alias
17-1A, 323/A3, CD326, CO-17A, CO17-1A, EGP, EGP-2, EGP34, EGP40, ESA, Ep-CAM, GA733-2, HEA125, KS1/4, KSA, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1, hEGP-2
Gene Description
epithelial cell adhesion molecule
Omim ID
185535Gene Ontology
HyperlinkGene Summary
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq
Other Designations
adenocarcinoma-associated antigen|carcinoma-associated antigen GA733-2|human epithelial glycoprotein-2|membrane component, chromosome 4, surface marker (35kD glycoprotein)|tumor-associated calcium signal transducer 1
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Interactome
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Disease
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