Camelid VHH recombinant monoclonal antibody cocktail (Biotin)
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Flow Cytometry
VHH-based CAR-T cells were sorted by MACS with Camelid VHH recombinant monoclonal antibody cocktail (Biotin)(Cat # RAB02385) and anti-Biotin magnetic nanobeads (Miltenyi) where naïve T cells serves as negative control. Then, the CAR- T cells were stained with Camelid VHH recombinant monoclonal antibody cocktail (iFluor 555) (Cat # RAB02387) and analyzed by FACS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody cocktail raised against camelid VHH antibody (llama, alpaca, and camel).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against llama VHH antibody
Reactivity
Camelid
Specificity
Rabbit Anti-Camelid VHH Antibody cocktail is specific for camelid VHH antibody (llama, alpaca, and camel). It has no cross-reactivity with mouse, rat, rabbit, goat or human immunoglobulins.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Recommend Usage
ELISA (1:5,000-1:10,000)
Flow Cytometry (1:50-1:800)
Magnetic-activated cell sorting (1:25-1:100)
Western blot (1:1,000-1:5,000)
The following concentration ranges are recommended starting points for this product.
Optimal working dilutions must be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10 mg/ml BSA, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C.
This product is stable for 1 year upon receipt, when handled and stored as instructed.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
VHH-based CAR-T cells were sorted by MACS with Camelid VHH recombinant monoclonal antibody cocktail (Biotin)(Cat # RAB02385) and anti-Biotin magnetic nanobeads (Miltenyi) where naïve T cells serves as negative control. Then, the CAR- T cells were stained with Camelid VHH recombinant monoclonal antibody cocktail (iFluor 555) (Cat # RAB02387) and analyzed by FACS.
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