Camelid VHH recombinant monoclonal antibody cocktail (HRP)
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More Files
- More Functions
Western Blot
Western Blot analysis of CD8A VHH-hIgG1 humanized monoclonal antibody, clone B (RAB01120-M03J).
Lane 1: Goat Anti-Human Ig FC HRP (5000X).
Lane 2: Camelid VHH recombinant monoclonal antibody cocktail (HRP) (1000X)(Cat # RAB02384).
Western Blot (Recombinant protein)
Western Blot analysis of CD8A Protein (0.2ug) with exposure time (A) 30 seconds (B) 90 seconds.
Lane 1: (1st Ab) Mouse Anti-HIS clone HIS.H8 (1ug/ml) and (2nd Ab) Jackson anti-mouse IgG-HRP (5000X).
Lane 2: (1st Ab) 2% milk in 0.2% PBST and (2nd Ab) Goat Anti-Human Ig FC HRP (5000X)
Lane 3: (1st Ab) CD8A VHH-hIgG1 humanized monoclonal antibody, clone B5 (1ug) and (2nd Ab) Goat Anti-Human Ig FC HRP (5000X)
Lane 4: (1st Ab) CD8A VHH-hIgG1 humanized monoclonal antibody, clone B5 (1ug) and (2nd Ab) Camelid VHH recombinant monoclonal antibody cocktail (HRP) (1000X)(Cat # RAB02384).
Sandwich ELISA is developed by using Camelid VHH recombinant monoclonal antibody, clone 96A3F5 (Cat # RAB02367) and Camelid VHH recombinant monoclonal antibody cocktail (HRP)(Cat # RAB02384) as the capture and detection antibodies, respectively.
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Specification
Product Description
Rabbit recombinant monoclonal antibody cocktail raised against camelid VHH antibody (llama, alpaca, and camel).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against llama VHH antibody
Reactivity
Camelid
Specificity
Rabbit Anti-Camelid VHH Antibody cocktail is specific for camelid sdAb (llama and camel). It has no cross-reactivity with mouse, rat, rabbit, goat or human immunoglobulins.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG
Quality Control Testing
Standard Curve
Sandwich ELISA is developed by using Camelid VHH recombinant monoclonal antibody, clone 96A3F5 (Cat # RAB02367) and Camelid VHH recombinant monoclonal antibody cocktail (HRP)(Cat # RAB02384) as the capture and detection antibodies, respectively.
Recommend Usage
ELISA (1:5,000-1:10,000)
Sandwich ELISA
Western blot (1:1,000-1:5,000)
The following concentration ranges are recommended starting points for this product.
Optimal working dilutions must be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10 mg/mL BSA, 0.01% thimerosal and 50% glycerol)
Storage Instruction
Store at -20°C.
This product is stable for 1 year upon receipt, when handled and stored as instructed.Note
This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of CD8A VHH-hIgG1 humanized monoclonal antibody, clone B (RAB01120-M03J).
Lane 1: Goat Anti-Human Ig FC HRP (5000X).
Lane 2: Camelid VHH recombinant monoclonal antibody cocktail (HRP) (1000X)(Cat # RAB02384).
Western Blot
Western Blot (Recombinant protein)
Western Blot analysis of CD8A Protein (0.2ug) with exposure time (A) 30 seconds (B) 90 seconds.
Lane 1: (1st Ab) Mouse Anti-HIS clone HIS.H8 (1ug/ml) and (2nd Ab) Jackson anti-mouse IgG-HRP (5000X).
Lane 2: (1st Ab) 2% milk in 0.2% PBST and (2nd Ab) Goat Anti-Human Ig FC HRP (5000X)
Lane 3: (1st Ab) CD8A VHH-hIgG1 humanized monoclonal antibody, clone B5 (1ug) and (2nd Ab) Goat Anti-Human Ig FC HRP (5000X)
Lane 4: (1st Ab) CD8A VHH-hIgG1 humanized monoclonal antibody, clone B5 (1ug) and (2nd Ab) Camelid VHH recombinant monoclonal antibody cocktail (HRP) (1000X)(Cat # RAB02384).
Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA
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