Anti-Camelid (Humanized VHH) Affinity Resin FF
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More Files
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Purification
Purification of Humanized VHH protein from E. coli lysate. Lanes: (1) Protein marker, (2) Whole cell lysate, (3) Flow through (4) Eluate of target Humanized VHH signal domain antibody-1 (M.W.=15 kDa).
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Specification
Product Description
Anti-Camelid (Humanized VHH) Affinity Resin FF (Fast Flow) is designed for purification of humanized VHH single domain antibody (sdAb) from commonly used protein expression systems such as bacteria, yeast and mammalian cells.
Product content
50% settled resin in TBS
Matrix
4% highly cross-linked agarose
Bead Size
90 μm
Ligand
Anti-Humanized VHH Antibody
Binding Capacity
Approximately 2 mg Humanized VHH per mL settled resin (depending on amino acid sequence of different Humanized VHHs)
Resin reuse
When stored at 2-8°C, the resin can be recycled at least 5 times with no loss of binding capability. While, it can be reused up to 10 times with minimum loss of binding capability.
Elution method
Acid buffer elution (pH 2.5)
Regulatory Status
For research use only (RUO)
Storage Buffer
In TBS (containing 0.02% sodium azide).
Storage Instruction
Store at 2-8°C for up to 12 months. Do not freeze the resin.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Purification
Purification of Humanized VHH protein from E. coli lysate. Lanes: (1) Protein marker, (2) Whole cell lysate, (3) Flow through (4) Eluate of target Humanized VHH signal domain antibody-1 (M.W.=15 kDa).
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