Anti-Camelid (Non-Humanized VHH) Affinity Resin
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More Files
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Purification
Purification of Camelid VHH from E. coli lysate. Lanes: (1) Protein marker, (2) Whole cell lysate, (3) Flow through (4) Washing eluate(first time), (5) Washing eluate(final-time), (6) Eluate of target Camelid VHH(M.W.=15 kDa monomer and 30 kDa dimer).
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Specification
Product Description
Anti-Camelid (Non-Humanized VHH) Affinity Resin is designed for purification of Camelid VHH single domain antibody (sdAb) from commonly used protein expression systems such as bacteria, yeast and mammalian cells.
Product content
50% settled resin in TBS
Matrix
4% cross-linked agarose
Bead Size
90 μm
Ligand
Rabbit Anti-Camelid VHH Antibody, mAb
Binding Capacity
Approximately 2-4 mg Camelid VHH protein per mL settled resin (depending on amino acid sequence of different Camelid VHHs)
Resin reuse
When stored at 2-8°C, the resin can be recycled at least 5 times with no loss of binding capability. The resin can be reused up to 10 times with minimum loss of binding capability.
Elution method
Acid buffer elution (pH 2.5)
Regulatory Status
For research use only (RUO)
Storage Buffer
In TBS (containing 0.02% sodium azide).
Storage Instruction
Store at 2-8°C for up to 12 months. Do not freeze the resin.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Purification
Purification of Camelid VHH from E. coli lysate. Lanes: (1) Protein marker, (2) Whole cell lysate, (3) Flow through (4) Washing eluate(first time), (5) Washing eluate(final-time), (6) Eluate of target Camelid VHH(M.W.=15 kDa monomer and 30 kDa dimer).
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