Recombinant rabbit monoclonal antibody raised against human IL2RA.
Original antibody is raised against cultured T-cells derived from a patient with Mycosis fungoides.
This antibody binds to p55 chain of IL2R on human T cells, Tac antigen, which is expressed on T cells activated by mitogens, soluble antigens, and alloantigens but not on resting T cells, thymocytes, B cells, monocytes, activated B cells, leukemic T cell blasts from patients with acute lymphoblastic leukemia, and long-term T cell lines.
Protein A affinity purification
Flow Cytometry (1 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.2 (0.02% Proclin 300).
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. [provided by RefSeq