IL2RA recombinant monoclonal antibody, clone AHT107
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More Files
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Immunofluorescence
Immunofluorescent staining of Jurkat cells with IL2RA recombinant monoclonal antibody, clone AHT107 (Cat # RAB03579).
Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix™ coverslips and stained with the chimeric r version of RAB03579 at 10 ug/mL followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with anti-Fluorescein antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03579
(B) DAPI
(C) Merged channels
(D) Isotype control
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human IL2RA.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against human T lymphoblasts.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
Blocking
ELISA
Flow Cytometry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of Jurkat cells with IL2RA recombinant monoclonal antibody, clone AHT107 (Cat # RAB03579).
Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix™ coverslips and stained with the chimeric r version of RAB03579 at 10 ug/mL followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with anti-Fluorescein antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03579
(B) DAPI
(C) Merged channels
(D) Isotype controlEnzyme-linked Immunoabsorbent Assay
Blocking
Flow Cytometry
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Gene Info — IL2RA
Entrez GeneID
3559Gene Name
IL2RA
Gene Alias
CD25, IDDM10, IL2R, TCGFR
Gene Description
interleukin 2 receptor, alpha
Gene Ontology
HyperlinkGene Summary
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. [provided by RefSeq
Other Designations
Interleukin-2 receptor|OTTHUMP00000019030|OTTHUMP00000044870|interleukin 2 receptor, alpha chain
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Interactome
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Pathway
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Disease
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