IL2RA recombinant monoclonal antibody, clone Basiliximab
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More Files
- More Functions
Western Blot
Western Blot analysis were resolved on a 10% SDS PAGE gel and blots probed at 1 ug/mL before being detected by a secondary antibody.
Immunohistochemistry
Immunohistochemistry analysis of Formalin fixed rat spleen slices were were stained at 5 ug/mL. The anti-human CD25 antibody also shows some cross-reactivity with rat in IHC.
Immunofluorescence
Immunofluorescence analysis of formaldehyde-fixed rat thymus slices were stained at 5 ug/mL and detected with a FITC-conjugated secondary antibody.
Flow Cytometry
Flow-cytometry were stained with an isotype control at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
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Specification
Product Description
Mouse recombinant monoclonal antibody raised against human IL-2R.
Antibody Species
Mouse
Immunogen
Original antibody is raised against by immunizing BALB/c mice with CTC 16 cells
Reactivity
Human
Specificity
Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit – binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R (αβγc) - T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG1, Kappa
Recommend Usage
Flow cytometry
Immunofluorescence
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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Applications
Western Blot
Western Blot analysis were resolved on a 10% SDS PAGE gel and blots probed at 1 ug/mL before being detected by a secondary antibody.Immunohistochemistry
Immunohistochemistry analysis of Formalin fixed rat spleen slices were were stained at 5 ug/mL. The anti-human CD25 antibody also shows some cross-reactivity with rat in IHC.Immunofluorescence
Immunofluorescence analysis of formaldehyde-fixed rat thymus slices were stained at 5 ug/mL and detected with a FITC-conjugated secondary antibody.Flow Cytometry
Flow-cytometry were stained with an isotype control at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer. -
Gene Info — IL2RA
Entrez GeneID
3559Protein Accession#
P01589Gene Name
IL2RA
Gene Alias
CD25, IDDM10, IL2R, TCGFR
Gene Description
interleukin 2 receptor, alpha
Gene Ontology
HyperlinkGene Summary
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. [provided by RefSeq
Other Designations
Interleukin-2 receptor|OTTHUMP00000019030|OTTHUMP00000044870|interleukin 2 receptor, alpha chain
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Interactome
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Pathway
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Disease
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