IL2RA recombinant monoclonal antibody, clone Daclizumab
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More Files
- More Functions
Western Blot
Western Blot analysis were resolved on a 10% SDS PAGE gel and blots probed at 1.5 ug/mL before being detected by a secondary antibody. The expected band size (31 kDa) is seen in the human-derived sample, as well as further bands that are specific to human.
Immunohistochemistry
Immunohistochemistry analysis of formalin fixed rat spleen slices were were stained at 5 ug/mL.
Flow Cytometry
Flow-cytometry were stained with an isotype control at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
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Specification
Product Description
Mouse recombinant monoclonal antibody raised against human IL-2R.
Antibody Species
Mouse
Immunogen
Original antibody is raised against cultured T-cells derived from a patient with Mycosis fungoides.
Reactivity
Human
Specificity
Binds to p55 chain of IL2R on human T cells, Tac antigen, which is expressed on T cells activated by mitogens, soluble antigens, and alloantigens but not on resting T cells, thymocytes, B cells, monocytes, activated B cells, leukemic T cell blasts from patients with acute lymphoblastic leukemia, and long-term T cell lines.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG1, Kappa
Recommend Usage
Flow cytometry
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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Applications
Western Blot
Western Blot analysis were resolved on a 10% SDS PAGE gel and blots probed at 1.5 ug/mL before being detected by a secondary antibody. The expected band size (31 kDa) is seen in the human-derived sample, as well as further bands that are specific to human.Immunohistochemistry
Immunohistochemistry analysis of formalin fixed rat spleen slices were were stained at 5 ug/mL.Flow Cytometry
Flow-cytometry were stained with an isotype control at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer. -
Gene Info — IL2RA
Entrez GeneID
3559Protein Accession#
P01589Gene Name
IL2RA
Gene Alias
CD25, IDDM10, IL2R, TCGFR
Gene Description
interleukin 2 receptor, alpha
Gene Ontology
HyperlinkGene Summary
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. [provided by RefSeq
Other Designations
Interleukin-2 receptor|OTTHUMP00000019030|OTTHUMP00000044870|interleukin 2 receptor, alpha chain
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Interactome
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Pathway
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Disease
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