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Last updated: 2016/10/23
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CDK9 polyclonal antibody

  • Catalog # : PAB9941
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of CDK9.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus and N-terminus of human CDK9.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 43
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • Antiserum will specifically react with a 43 KDa cdk9 (PITALRE) protein from human, rat and mouse tissue. Cross reactivity with cdk9 (PITALRE) from other species may also occur. The murine cDNA is shown to be 98% identical with human.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:50000)
    Western Blot (1:500-1:3000)
    Immunohistochemistry (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • 1.
  • Binding of CDK9 to TRAF2.
    MacLachlan TK, Sang N, De Luca A, Puri PL, Levrero M, Giordano A.J Cell Biochem. 1998 Dec 15;71(4):467-78.
  • Applications
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry
  • Immunocytochemical staining of mouse tissue using CDK9 polyclonal antibody (Cat # PAB9941).
    The staining shows the location of CDK9 protein in developing mouse tissue. Arrows indicate areas of high expression.
    Panel A : Peroxidase-DAB immunostaining of CDK9 protein in the developing mouse brain in the differentiated region of the medulla oblongata just below the fourth ventricle.
    Similar staining is shown in Panel B in the dorsal root ganglia.
    Panel C : Fluorescein immunofluorescence of CDK9 in skeletal muscle.
    Similar staining is shown in Panel D in cardiac muscle.
  • Immunoprecipitation
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 1025
  • Protein Accession#:
  • P50750;NP_001252
  • Gene Name:
  • CDK9
  • Gene Alias:
  • Gene Description:
  • cyclin-dependent kinase 9
  • Gene Summary:
  • The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq
  • Other Designations:
  • CDC2-related kinase,OTTHUMP00000022198,cell division protein kinase 9,serine/threonine protein kinase PITALRE
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