CDK9 (phospho T29) polyclonal antibody
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More Files
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Western Blot (Recombinant protein)
Western blot using CDK9 (phospho T29) polyclonal antibody (Cat # PAB10000) shows detection of phospho-CDK9.
100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29.
The primary antibody was used at a 1:1000 dilution.
Personal Communication, J. Brady, NCI, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDK9.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T29 of human CDK9.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to human CDK9 protein phosphorylated at T29.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:24000)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot using CDK9 (phospho T29) polyclonal antibody (Cat # PAB10000) shows detection of phospho-CDK9.
100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29.
The primary antibody was used at a 1:1000 dilution.
Personal Communication, J. Brady, NCI, Bethesda, MD.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDK9
Entrez GeneID
1025Protein Accession#
NP_001252;P50750Gene Name
CDK9
Gene Alias
C-2k, CDC2L4, CTK1, PITALRE, TAK
Gene Description
cyclin-dependent kinase 9
Omim ID
603251Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq
Other Designations
CDC2-related kinase|OTTHUMP00000022198|cell division protein kinase 9|serine/threonine protein kinase PITALRE
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Interactome
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Publication Reference
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Tax interacts with P-TEFb in a novel manner to stimulate human T-lymphotropic virus type 1 transcription.
Zhou M, Lu H, Park H, Wilson-Chiru J, Linton R, Brady JN.
Journal of Virology 2006 May; 80(10):4781.
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The bromodomain protein Brd4 is a positive regulatory component of P-TEFb and stimulates RNA polymerase II-dependent transcription.
Jang MK, Mochizuki K, Zhou M, Jeong HS, Brady JN, Ozato K.
Molecular Cell 2005 Aug; 19(4):523.
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Differential localization and expression of the Cdk9 42k and 55k isoforms.
Liu H, Herrmann CH.
Journal of Cellular Physiology 2005 Apr; 203(1):251.
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Tax interacts with P-TEFb in a novel manner to stimulate human T-lymphotropic virus type 1 transcription.
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