CDK9 recombinant monoclonal antibody, clone R08-4F6
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More Files
- More Functions
Western Blot
Western blot analysis of Cdc37 in MCF-7, HepG2, U2OS, Lncap lysates using Cdc37 antibody.
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with CDK9 recombinant monoclonal antibody, clone R08-4F6 (Cat # RAB01473).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CDK9.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human CDK9.
Theoretical MW (kDa)
Calculated MW: 43 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Cdc37 in MCF-7, HepG2, U2OS, Lncap lysates using Cdc37 antibody.Immunocytochemistry
Immunocytochemistry staining of HeLa cells with CDK9 recombinant monoclonal antibody, clone R08-4F6 (Cat # RAB01473).Immunofluorescence
Immunoprecipitation
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Gene Info — CDK9
Entrez GeneID
1025Protein Accession#
P50750Gene Name
CDK9
Gene Alias
C-2k, CDC2L4, CTK1, PITALRE, TAK
Gene Description
cyclin-dependent kinase 9
Omim ID
603251Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq
Other Designations
CDC2-related kinase|OTTHUMP00000022198|cell division protein kinase 9|serine/threonine protein kinase PITALRE
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Interactome
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