CDK9 (phospho T186) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of 3T3 cell lysate.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of human CDK9 (phospho T186).
Immunogen
A synthetic peptide corresponding to amino acids 130-210 of human CDK9 (phospho T186).
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of Cdk9 protein only when phosphorylated at T186.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
ELISA (1:5000)
Western Blot (1:500-2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.5% BSA and 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of 3T3 cell lysate.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDK9
Entrez GeneID
1025Protein Accession#
P50750Gene Name
CDK9
Gene Alias
C-2k, CDC2L4, CTK1, PITALRE, TAK
Gene Description
cyclin-dependent kinase 9
Omim ID
603251Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq
Other Designations
CDC2-related kinase|OTTHUMP00000022198|cell division protein kinase 9|serine/threonine protein kinase PITALRE
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