LAT polyclonal antibody
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More Files
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Western Blot (Cell lysate)
LAT polyclonal antibody (Cat # PAB6652) (0.01 ug/mL) staining of peripheral blood mononucleocytes lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of LAT.
Immunogen
A synthetic peptide corresponding to human LAT.
Sequence
C-GAPDYENLQELN
Host
Goat
Theoretical MW (kDa)
27.9, 25, 24.8
Reactivity
Human, Mouse, Rat
Specificity
This antibody is expected to recognize isoform a (NP_055202.1), isoform b (NP_001014987.1 and NP_001014989.1) and isoform c (NP_001014988.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
LAT polyclonal antibody (Cat # PAB6652) (0.01 ug/mL) staining of peripheral blood mononucleocytes lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — LAT
Entrez GeneID
27040Protein Accession#
NP_055202.1;NP_001014987.1;NP_001014989.1;NP_001014988.1Gene Name
LAT
Gene Alias
LAT1, pp36
Gene Description
linker for activation of T cells
Omim ID
602354Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
36 kDa phospho-tyrosine adaptor protein
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Interactome
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Pathway
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Publication Reference
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LAT is required for TCR-mediated activation of PLCgamma1 and the Ras pathway.
Finco TS, Kadlecek T, Zhang W, Samelson LE, Weiss A.
Immunity 1998 Nov; 9(5):617.
Application：WB-Ce, WB-Tr, Human, Jurkat, J.CaM2 cells.
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LAT is required for TCR-mediated activation of PLCgamma1 and the Ras pathway.
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