LAT (phospho Y191) recombinant monoclonal antibody, clone 2E4
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More Files
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Western Blot
Western blot analysis of Lane 1:Jurkat whole cell lysate (treated with EGF 100ng/ml/20mins), Lane 2: Jurkat whole cell lysate (not treated), Lane 3: Hela whole cell lysate (treated with Pervanadate), Lane 4: Hela whole cell lysate (not treated), Lane 5: HepG2 whole cell lysate (treated with EGF 100ng/ml/20mins) and Lane 6: HepG2 whole cell lysate (not treated) with LAT (phospho Y191) recombinant monoclonal antibody, clone 2E4 (Cat # RAB04296).
Immunohistochemistry
Immunohistochemical staining of human colon cancer with LAT (phospho Y191) recombinant monoclonal antibody, clone 2E4 (Cat # RAB04296) (diluated at 1:100)
Immunohistochemistry
Immunohistochemical staining of human tonsil tissue with LAT (phospho Y191) recombinant monoclonal antibody, clone 2E4 (Cat # RAB04296) (diluated at 1:100).
Immunofluorescence
Immunofluorescent staining of Hela cells with LAT (phospho Y191) recombinant monoclonal antibody, clone 2E4 (Cat # RAB04296) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Immunoprecipitation
Immunoprecipitation analysis of A549 cell lysate with LAT (phospho Y191) recombinant monoclonal antibody, clone 2E4 (Cat # RAB04296).
Lane 1: rabbit control IgG, Lane 2: RAB04296 precipitates and Lane 3: Input (A549 whole cell lysates).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human LAT.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Y191 of human LAT.
Theoretical MW (kDa)
Calculated MW: 38 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:Jurkat whole cell lysate (treated with EGF 100ng/ml/20mins), Lane 2: Jurkat whole cell lysate (not treated), Lane 3: Hela whole cell lysate (treated with Pervanadate), Lane 4: Hela whole cell lysate (not treated), Lane 5: HepG2 whole cell lysate (treated with EGF 100ng/ml/20mins) and Lane 6: HepG2 whole cell lysate (not treated) with LAT (phospho Y191) recombinant monoclonal antibody, clone 2E4 (Cat # RAB04296).Immunohistochemistry
Immunohistochemical staining of human colon cancer with LAT (phospho Y191) recombinant monoclonal antibody, clone 2E4 (Cat # RAB04296) (diluated at 1:100)Immunohistochemistry
Immunohistochemical staining of human tonsil tissue with LAT (phospho Y191) recombinant monoclonal antibody, clone 2E4 (Cat # RAB04296) (diluated at 1:100).Immunofluorescence
Immunofluorescent staining of Hela cells with LAT (phospho Y191) recombinant monoclonal antibody, clone 2E4 (Cat # RAB04296) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Immunoprecipitation
Immunoprecipitation analysis of A549 cell lysate with LAT (phospho Y191) recombinant monoclonal antibody, clone 2E4 (Cat # RAB04296).
Lane 1: rabbit control IgG, Lane 2: RAB04296 precipitates and Lane 3: Input (A549 whole cell lysates).Enzyme-linked Immunoabsorbent Assay
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Gene Info — LAT
Entrez GeneID
27040Protein Accession#
O43561Gene Name
LAT
Gene Alias
LAT1, pp36
Gene Description
linker for activation of T cells
Omim ID
602354Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
36 kDa phospho-tyrosine adaptor protein
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Interactome
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Pathway
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