LAT monoclonal antibody, clone LAT-01
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More Files
- More Functions
Flow Cytometry
Flow Cytometry analysis of LAT expression in human peripheral blood lymphocytes (PBL) using LAT monoclonal antibody, clone LAT-01 (Cat # MAB3865) (surface markers were stained prior to intracellular staining of human LAT).
Panel A : gate on CD4+ cells.
Panel B : gate on CD8+ cells.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant LAT.
Immunogen
Recombinant protein corresponding to cytoplasmic region of human LAT.
Host
Mouse
Theoretical MW (kDa)
36-38
Reactivity
Human
Specificity
This antibody reacts with Linker for activation of T cells (LAT), a 36-38 KDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunoprecipitation
Flow Cytometry
Flow Cytometry analysis of LAT expression in human peripheral blood lymphocytes (PBL) using LAT monoclonal antibody, clone LAT-01 (Cat # MAB3865) (surface markers were stained prior to intracellular staining of human LAT).
Panel A : gate on CD4+ cells.
Panel B : gate on CD8+ cells. -
Gene Info — LAT
Entrez GeneID
27040Gene Name
LAT
Gene Alias
LAT1, pp36
Gene Description
linker for activation of T cells
Omim ID
602354Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
36 kDa phospho-tyrosine adaptor protein
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Interactome
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Pathway
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Publication Reference
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LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival.
Yamasaki S, Ishikawa E, Sakuma M, Kanagawa O, Cheng AM, Malissen B, Saito T.
Molecular and Cellular Biology 2007 Jun; 27(12):4406.
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Transmembrane adaptor molecules: a new category of lymphoid-cell markers.
Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rudiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsi V.
Blood 2005 Sep; 107(1):213.
Application：IHC-P, Human, Rat, Human normal and neoplastic lymphoid tissue, Rat appendix, plasma cells, thymus, tonsil.
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Right time, right place: the organization of membrane proximal signaling.
Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA.
Seminars in Immunology 2005 Feb; 17(1):35.
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LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival.
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