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Last updated: 2016/11/27
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UBE2I polyclonal antibody

  • Catalog # : PAB6303
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of UBE2I.
  • Immunogen:
  • A synthetic peptide corresponding to N-terminus of human UBE2I.
  • Sequence:
  • SGIALSRLAQERK-C
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 18
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:64000)
    Western Blot (0.1-0.3 ug/mL)
    Immunohistochemistry (1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • UBE2I polyclonal antibody (Cat # PAB6303) staining (0.1 ug/mL) of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • UBE2I polyclonal antibody (Cat # PAB6303) staining (0.3 ug/mL) of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 7329
  • Protein Accession#:
  • NP_003336.1;NP_919235.1;NP_919236.1;NP_919237.1
  • Gene Name:
  • UBE2I
  • Gene Alias:
  • C358B7.1,P18,UBC9
  • Gene Description:
  • ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
  • Gene Summary:
  • The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
  • SUMO-1-protein ligase,ubiquitin carrier protein,ubiquitin conjugating enzyme 9,ubiquitin-conjugating enzyme E2I,ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9),ubiquitin-conjugating enzyme UbcE2A,ubiquitin-like protein SUMO-1 conjugating enzym
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