BUB1B polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BUB1B.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human BUB1B.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of BUB1B (arrow) using rabbit BUB1B polyclonal antibody (Cat # PAB3263). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BUB1B gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with BUB1B polyclonal antibody (Cat # PAB3263) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — BUB1B
Entrez GeneID
701Protein Accession#
O60566Gene Name
BUB1B
Gene Alias
BUB1beta, BUBR1, Bub1A, MAD3L, SSK1, hBUBR1
Gene Description
budding uninhibited by benzimidazoles 1 homolog beta (yeast)
Gene Ontology
HyperlinkGene Summary
This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq
Other Designations
BUB1 budding uninhibited by benzimidazoles 1 homolog beta|MAD3/BUB1-related protein kinase|budding uninhibited by benzimidazoles 1, beta
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Interactome
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Pathway
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Disease
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Publication Reference
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Characterization of MAD2B and other mitotic spindle checkpoint genes.
Cahill DP, da Costa LT, Carson-Walter EB, Kinzler KW, Vogelstein B, Lengauer C.
Genomics 1999 Jun; 58(2):181.
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The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner.
Davenport JW, Fernandes ER, Harris LD, Neale GA, Goorha R.
Genomics 1999 Jan; 55(1):113.
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Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1.
Chan GK, Schaar BT, Yen TJ.
The Journal of Cell Biology 1998 Oct; 143(1):49.
Application:IF, IP-WB, WB-Ce, Human, HeLa cells.
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Characterization of MAD2B and other mitotic spindle checkpoint genes.
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