BUB1B recombinant monoclonal antibody, clone 7H4
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: K562 whole cell lysate; Lane 2: U-251 whole cell lysate.
Flow Cytometry
Overlay histogram showing Hela cells stained with BUB1B recombinant monoclonal antibody, clone 7H4 (red line) at 1:50.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human BUB1B.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human BUB1B.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry (1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562 whole cell lysate; Lane 2: U-251 whole cell lysate.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay histogram showing Hela cells stained with BUB1B recombinant monoclonal antibody, clone 7H4 (red line) at 1:50. -
Gene Info — BUB1B
Entrez GeneID
701Protein Accession#
O60566Gene Name
BUB1B
Gene Alias
BUB1beta, BUBR1, Bub1A, MAD3L, SSK1, hBUBR1
Gene Description
budding uninhibited by benzimidazoles 1 homolog beta (yeast)
Gene Ontology
HyperlinkGene Summary
This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq
Other Designations
BUB1 budding uninhibited by benzimidazoles 1 homolog beta|MAD3/BUB1-related protein kinase|budding uninhibited by benzimidazoles 1, beta
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Interactome
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Pathway
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Disease
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