BUB1B recombinant monoclonal antibody, clone R05-3G7
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More Files
- More Functions
Western Blot
Western blot analysis of BubR1 in CHO-K1, Ramos lysates using BUBR1 antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human BUB1B.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human BUB1B.
Theoretical MW (kDa)
Calculated MW: 120 k
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of BubR1 in CHO-K1, Ramos lysates using BUBR1 antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
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Gene Info — BUB1B
Entrez GeneID
701Protein Accession#
O60566Gene Name
BUB1B
Gene Alias
BUB1beta, BUBR1, Bub1A, MAD3L, SSK1, hBUBR1
Gene Description
budding uninhibited by benzimidazoles 1 homolog beta (yeast)
Gene Ontology
HyperlinkGene Summary
This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq
Other Designations
BUB1 budding uninhibited by benzimidazoles 1 homolog beta|MAD3/BUB1-related protein kinase|budding uninhibited by benzimidazoles 1, beta
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Interactome
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Pathway
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Disease
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