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Last updated: 2016/10/23
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RAD21 polyclonal antibody

  • Catalog # : PAB21266
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against recombinant RAD21.
  • Immunogen:
  • Recombinant protein corresponding to amino acids of human RAD21.
  • Sequence:
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunohistochemistry (1:500-1:1000)
    Immunofluorescence (1-4 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of human small intestine with RAD21 polyclonal antibody (Cat # PAB21266) shows strong nuclear positivity in glandular cells.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of human cell line U-251MG with RAD21 polyclonal antibody (Cat # PAB21266) at 1-4 ug/mL dilution shows positivity in nuclei but not nucleoli.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5885
  • Gene Name:
  • RAD21
  • Gene Alias:
  • FLJ25655,FLJ40596,HR21,HRAD21,KIAA0078,MCD1,NXP1,SCC1,hHR21
  • Gene Description:
  • RAD21 homolog (S. pombe)
  • Gene Summary:
  • The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq
  • Other Designations:
  • RAD21 homolog,nuclear matrix protein 1,protein involved in DNA double-strand break repair
  • Gene Pathway
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