RAD21 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human RAD21.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human RAD21.
Theoretical MW (kDa)
110
Reactivity
Hamster, Human
Specificity
Recognizes endogenous levels of RAD21 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Hela (A), CHOK1 (B), Ramos (C) whole cell lysates with RAD21 recombinant monoclonal antibody (Cat # RAB02605).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using RAD21 recombinant monoclonal antibody (Cat # RAB02605). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.97). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of HeLa cells with RAD21 recombinant monoclonal antibody (Cat # RAB02605). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. -
Gene Info — RAD21
Entrez GeneID
5885Protein Accession#
O60216Gene Name
RAD21
Gene Alias
FLJ25655, FLJ40596, HR21, HRAD21, KIAA0078, MCD1, NXP1, SCC1, hHR21
Gene Description
RAD21 homolog (S. pombe)
Omim ID
606462Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq
Other Designations
RAD21 homolog|nuclear matrix protein 1|protein involved in DNA double-strand break repair
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Interactome
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Pathway
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Disease
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