RAD21 monoclonal antibody, clone 1B6D1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell; Lane 2: HEK293 cell; Lane 3: K562 cell; Lane 4: C6 cell; Lane 5: COS-7 cell; Lane 6: NIH/3T3 cell with RAD21 monoclonal antibody.
Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) RAD21-hIgGFc transfected HEK293 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with RAD21 monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with RAD21 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAD21 monoclonal antibody, clone 1B6D1.
Flow Cytometry
Flow cytometric analysis of HeLa cells with RAD21 monoclonal antibody (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human RAD21.
Immunogen
Recombinant protein corresponding to amino acid 287-403 of human RAD21 from E. coli.
Host
Mouse
Theoretical MW (kDa)
71.7kDa
Reactivity
Human, Monkey, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunocytochemistry (1:200-1:1000)
Flow Cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell; Lane 2: HEK293 cell; Lane 3: K562 cell; Lane 4: C6 cell; Lane 5: COS-7 cell; Lane 6: NIH/3T3 cell with RAD21 monoclonal antibody.Western Blot (Transfected lysate)
Western Blot analysis of (1) HEK293 cells, (2) RAD21-hIgGFc transfected HEK293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissues with RAD21 monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with RAD21 monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor- 555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of RAD21 monoclonal antibody, clone 1B6D1.Flow Cytometry
Flow cytometric analysis of HeLa cells with RAD21 monoclonal antibody (green) and negative control (red). -
Gene Info — RAD21
Entrez GeneID
5885Gene Name
RAD21
Gene Alias
FLJ25655, FLJ40596, HR21, HRAD21, KIAA0078, MCD1, NXP1, SCC1, hHR21
Gene Description
RAD21 homolog (S. pombe)
Omim ID
606462Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq
Other Designations
RAD21 homolog|nuclear matrix protein 1|protein involved in DNA double-strand break repair
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Interactome
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Pathway
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Disease
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