RAD21 recombinant monoclonal antibody, clone R03-5I0
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More Files
- More Functions
Western Blot
Western blot analysis of Rad21 in Hela, CHO-K1, Ramos lysates using Rad21 antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with RAD21 recombinant monoclonal antibody, clone R03-5I0 (Cat # RAB01219).
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with RAD21 recombinant monoclonal antibody, clone R03-5I0 (Cat # RAB01219).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human RAD21.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against synthetic peptide corresponding to human Rad21.
Theoretical MW (kDa)
Calculated MW: 72 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine pH 7.4 (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Rad21 in Hela, CHO-K1, Ramos lysates using Rad21 antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung cancer with RAD21 recombinant monoclonal antibody, clone R03-5I0 (Cat # RAB01219).Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with RAD21 recombinant monoclonal antibody, clone R03-5I0 (Cat # RAB01219).Immunofluorescence
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Gene Info — RAD21
Entrez GeneID
5885Protein Accession#
O60216Gene Name
RAD21
Gene Alias
FLJ25655, FLJ40596, HR21, HRAD21, KIAA0078, MCD1, NXP1, SCC1, hHR21
Gene Description
RAD21 homolog (S. pombe)
Omim ID
606462Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq
Other Designations
RAD21 homolog|nuclear matrix protein 1|protein involved in DNA double-strand break repair
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Interactome
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Pathway
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Disease
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