MTOR polyclonal antibody
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More Files
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Immunoprecipitation
The cell lysate derived from MCF-7 was immuno-precipitated by MTOR polyclonal antibody (Cat # PAB12715), resolved onto 7.5% SDS-PAGE, transferred onto NC membrane, then immuno-blotted by MTOR polyclonal antibody (Cat # PAB12715) at 1 : 500.
An immunoreactive band around ~290 kDa was observed (lane 1). Lane 2 is a negative control.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MTOR.
Immunogen
A synthetic peptide corresponding to C-terminus of human MTOR.
Host
Rabbit
Theoretical MW (kDa)
290
Reactivity
Human, Mouse, Rat
Specificity
This antibody only recognizes ~290 KDa of human MTOR.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunoprecipitation
The cell lysate derived from MCF-7 was immuno-precipitated by MTOR polyclonal antibody (Cat # PAB12715), resolved onto 7.5% SDS-PAGE, transferred onto NC membrane, then immuno-blotted by MTOR polyclonal antibody (Cat # PAB12715) at 1 : 500.
An immunoreactive band around ~290 kDa was observed (lane 1). Lane 2 is a negative control.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MTOR
Entrez GeneID
2475Gene Name
MTOR
Gene Alias
FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Gene Description
mechanistic target of rapamycin
Omim ID
601231Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq
Other Designations
FK506 binding protein 12-rapamycin associated protein 1|FK506 binding protein 12-rapamycin associated protein 2|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP-rapamycin associated protein|FKBP12-rapamycin complex-associated protein 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Dissociation of raptor from mTOR is a mechanism of rapamycin-induced inhibition of mTOR function.
Oshiro N, Yoshino K, Hidayat S, Tokunaga C, Hara K, Eguchi S, Avruch J, Yonezawa K.
Genes to Cells: Devoted to Molecular & Cellular Mechanisms 2004 Apr; 9(4):359.
Application：IP, WB, Human, HEK 293 cells.
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Dissociation of raptor from mTOR is a mechanism of rapamycin-induced inhibition of mTOR function.
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