MTOR (phospho S2481) recombinant monoclonal antibody, clone 3H11
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More Files
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Western Blot
Western blot analysis of Lane 1:A549 whole cell lysate (not treated), Lane 2: A549 whole cell lysate (treated with EGF 100ng/ml/20mins), Lane 3: 293 whole cell lysate (not treated) and Lane 4: 293 whole cell lysate (treated with Calyculin A 100nM/60 mins) with MTOR (phospho S2481) recombinant monoclonal antibody, clone 3H11 (Cat # RAB04281).
Immunofluorescence
Immunofluorescent staining of Hela cells with MTOR (phospho S2481) recombinant monoclonal antibody, clone 3H11 (Cat # RAB04281) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MTOR.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S2481 of human MTOR.
Theoretical MW (kDa)
Calculated MW: 289 k
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:A549 whole cell lysate (not treated), Lane 2: A549 whole cell lysate (treated with EGF 100ng/ml/20mins), Lane 3: 293 whole cell lysate (not treated) and Lane 4: 293 whole cell lysate (treated with Calyculin A 100nM/60 mins) with MTOR (phospho S2481) recombinant monoclonal antibody, clone 3H11 (Cat # RAB04281).Immunofluorescence
Immunofluorescent staining of Hela cells with MTOR (phospho S2481) recombinant monoclonal antibody, clone 3H11 (Cat # RAB04281) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — MTOR
Entrez GeneID
2475Protein Accession#
P42345Gene Name
MTOR
Gene Alias
FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Gene Description
mechanistic target of rapamycin
Omim ID
601231Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq
Other Designations
FK506 binding protein 12-rapamycin associated protein 1|FK506 binding protein 12-rapamycin associated protein 2|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP-rapamycin associated protein|FKBP12-rapamycin complex-associated protein 1
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Interactome
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Pathway
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Disease
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