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Western Blot (Cell lysate)

Western blot using MTOR polyclonal antibody (Cat # PAB10278) shows detection of a band ~ 245 KDa corresponding to human MTOR (arrowhead).
Approximately 30 ug of HEK293 cell lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose.
After blocking, the membrane was probed with the primary antibody diluted to 1 : 650 for 2h at RT.
The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

2475

Protein Accession#

P42345

Gene Name

MTOR

Gene Alias

FRAP, FRAP1, FRAP2, RAFT1, RAPT1

Gene Description

mechanistic target of rapamycin

Omim ID

601231

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq

Other Designations

FK506 binding protein 12-rapamycin associated protein 1|FK506 binding protein 12-rapamycin associated protein 2|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP-rapamycin associated protein|FKBP12-rapamycin complex-associated protein 1