Western blot using MTOR polyclonal antibody (Cat # PAB10278) shows detection of a band ~ 245 KDa corresponding to human MTOR (arrowhead). Approximately 30 ug of HEK293 cell lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1 : 650 for 2h at RT. The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq
FK506 binding protein 12-rapamycin associated protein 1,FK506 binding protein 12-rapamycin associated protein 2,FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP-rapamycin associated protein,FKBP12-rapamycin complex-associated protein 1