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Last updated: 2016/10/23
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MTOR polyclonal antibody

  • Catalog # : PAB10278
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of MTOR.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 2440-2457 of human MTOR.
  • Host:
  • Rabbit
  • Reactivity:
  • Dog, Human, Mouse, Rat
  • Specificity:
  • Reactivity occurs with both phosphorylated and non-phosphorylated forms of mTOR at S2448 from human derived tissues and cells.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:4000-1:20000)
    Western Blot (1:250-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using MTOR polyclonal antibody (Cat # PAB10278) shows detection of a band ~ 245 KDa corresponding to human MTOR (arrowhead).
    Approximately 30 ug of HEK293 cell lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose.
    After blocking, the membrane was probed with the primary antibody diluted to 1 : 650 for 2h at RT.
    The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
    IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2475
  • Gene Name:
  • MTOR
  • Gene Alias:
  • Gene Description:
  • mechanistic target of rapamycin
  • Gene Summary:
  • The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq
  • Other Designations:
  • FK506 binding protein 12-rapamycin associated protein 1,FK506 binding protein 12-rapamycin associated protein 2,FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP-rapamycin associated protein,FKBP12-rapamycin complex-associated protein 1
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