Western blot using GSK3B (phospho S9) polyclonal antibody (Cat # PAB10055) at a 1:1,000 dilution. All lanes contain human 293T whole cell lysate showing a band at 47 KDa. Cells were serum starved for 24 h prior to extraction. Key : Lane 1, Control; Lane 2, treated with IGF-1 (100 ng/mL) for 20'; lane 3, pre-treated with 10 uM selective PI3K inhibitor and treated with IGF-1 (100 ng/mL) for 20'. Molecular weight markers confirm a MW of ~ 49 KDa. Use a 1 : 2,000 dilution of HRP Goat-a-Rabbit IgG for detection.
Immunohistochemistry of GSK3B (phospho S9) polyclonal antibody (Cat # PAB10055) was used at a 1 : 200 dilution to detect GSK3B by immunohistochemistry in human ovarian cancer tumor tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene