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Last updated: 2015/5/24
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GSK3B (phospho S9) polyclonal antibody

  • Catalog # : PAB10055
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of GSK3B.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S9 of human GSK3B.
  • Host:
  • Rabbit
  • Reactivity:
  • Chicken, Dog, Frog, Human, Rat, Zebra fish
  • Specificity:
  • This phospho specific polyclonal antibody reacts with phosphorylated pS9 of human GSK3B. Reactivity with non-phosphorylated human GSK3B is minimal.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:50000)
    Western Blot (1:500-1:3000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using GSK3B (phospho S9) polyclonal antibody (Cat # PAB10055) at a 1:1,000 dilution.
    All lanes contain human 293T whole cell lysate showing a band at 47 KDa.
    Cells were serum starved for 24 h prior to extraction.
    Key : Lane 1, Control; Lane 2, treated with IGF-1 (100 ng/mL) for 20'; lane 3, pre-treated with 10 uM selective PI3K inhibitor and treated with IGF-1 (100 ng/mL) for 20'.
    Molecular weight markers confirm a MW of ~ 49 KDa.
    Use a 1 : 2,000 dilution of HRP Goat-a-Rabbit IgG for detection.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of GSK3B (phospho S9) polyclonal antibody (Cat # PAB10055) was used at a 1 : 200 dilution to detect GSK3B by immunohistochemistry in human ovarian cancer tumor tissue.
    Tissue was formalin-fixed and paraffin embedded.
    Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.
  • Immunofluorescence
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Gene Information
  • Entrez GeneID:
  • 2932
  • Protein Accession#:
  • NM_002093(Human);P49841(Human)
  • Gene Name:
  • GSK3B
  • Gene Alias:
  • -
  • Gene Description:
  • glycogen synthase kinase 3 beta
  • Gene Summary:
  • The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene
  • Other Designations:
  • GSK3beta isoform,glycogen synthase kinase-3 beta
  • Interactome
  • Interactome
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