GSK3B (phospho Ser9) recombinant monoclonal antibody, clone 3A6
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate (treated with Calyculin A 100nM/60mins), Lane 2: Hela whole cell lysate (not treated), Lane 3: 293 whole cell lysate (treated with Calyculin A 100nM/60 mins), Lane 4: 293 whole cell lysate (not treated), Lane 5: A549 whole cell lysate (treated with Calyculin A 100nM/60 mins) and Lane 6: A549 whole cell lysate (not treated) with GSK3B (phospho Ser9) recombinant monoclonal antibody, clone 3A6 (Cat # RAB04284).
Immunohistochemistry
Immunohistochemical staining of human kidney tissue with GSK3B (phospho Ser9) recombinant monoclonal antibody, clone 3A6 (Cat # RAB04284) (diluated at 1:100).
Immunofluorescence
Immunofluorescent staining of Hela cells with GSK3B (phospho Ser9) recombinant monoclonal antibody, clone 3A6 (Cat # RAB04284) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human GSK3B.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Ser9 of human GSK3B.
Theoretical MW (kDa)
Calculated MW: 46 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate (treated with Calyculin A 100nM/60mins), Lane 2: Hela whole cell lysate (not treated), Lane 3: 293 whole cell lysate (treated with Calyculin A 100nM/60 mins), Lane 4: 293 whole cell lysate (not treated), Lane 5: A549 whole cell lysate (treated with Calyculin A 100nM/60 mins) and Lane 6: A549 whole cell lysate (not treated) with GSK3B (phospho Ser9) recombinant monoclonal antibody, clone 3A6 (Cat # RAB04284).Immunohistochemistry
Immunohistochemical staining of human kidney tissue with GSK3B (phospho Ser9) recombinant monoclonal antibody, clone 3A6 (Cat # RAB04284) (diluated at 1:100).Immunofluorescence
Immunofluorescent staining of Hela cells with GSK3B (phospho Ser9) recombinant monoclonal antibody, clone 3A6 (Cat # RAB04284) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — GSK3B
Entrez GeneID
2932Protein Accession#
P49841Gene Name
GSK3B
Gene Alias
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Gene Description
glycogen synthase kinase 3 beta
Omim ID
605004Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene
Other Designations
GSK3beta isoform|glycogen synthase kinase-3 beta
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Interactome
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Pathway
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Disease
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