LIF (Human) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
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Specification
Product Description
Human LIF (P15018, 23 a.a. - 202 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
SPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF
Host
Escherichia coli
Theoretical MW (kDa)
19.7
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 0.2 EU per 1 ug of protein (determined by gel clotting method)
Activity
ED50 < 0.2 ng/ml, measured by a cell differentiation assay using TF-1 cells, corresponding to a specific activity of > 5.0 x 106 units/mg.
Quality Control Testing
2 ug protein in Reducing and Non-Reducing SDS PAGE Stained with Coomassie Blue
Storage Buffer
Lyophilized from sterile distilled Water up to 100 ug/ml
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — LIF
Entrez GeneID
3976Protein Accession#
P15018Gene Name
LIF
Gene Alias
CDF, DIA, HILDA
Gene Description
leukemia inhibitory factor (cholinergic differentiation factor)
Omim ID
159540Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. [provided by RefSeq
Other Designations
D factor|cholinergic differentiation factor|differentiation inhibitory activity|differentiation stimulating factor
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Interactome
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