LIF (Human) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
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Specification
Product Description
Human LIF (P15018, 23 a.a. - 202 a.a.) partial length recombinant protein with His tag expressed in Baculovirus expression system.
Sequence
AQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF
Host
Viruses
Theoretical MW (kDa)
20.8
Form
Liquid
Preparation Method
Baculovirus expression system
Purity
> 90% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
ED50 is < 0.5 ng/mL, measured in a cell proliferation assay using TF-1 human erythroleukemic cell.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.
Storage Buffer
In Phosphate-Buffer Saline pH 7.4 (10% glycerol)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — LIF
Entrez GeneID
3976Protein Accession#
P15018Gene Name
LIF
Gene Alias
CDF, DIA, HILDA
Gene Description
leukemia inhibitory factor (cholinergic differentiation factor)
Omim ID
159540Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. [provided by RefSeq
Other Designations
D factor|cholinergic differentiation factor|differentiation inhibitory activity|differentiation stimulating factor
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Interactome
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