Il2ra monoclonal antibody, clone 7D4
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More Files
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Flow Cytometry
Cells from BALB/c spleen were activated by PMA (10 ng/mL) and ionomycin (400 ng/mL) for 48 hours, then stained with Il2ra monoclonal antibody, clone 7D4 (Cat # MAB3228)-FITC and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against Il2ra.
Immunogen
Native Il2ra from mouse helper T-cell clone HT-2.
Host
Rat
Reactivity
Mouse
Specificity
Low affinity alpha chain IL-2 receptor (IL-2Ralpha), Mr 55 KDa.
Form
Liquid
Isotype
IgM
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Immunoprecipitation
Flow Cytometry
Cells from BALB/c spleen were activated by PMA (10 ng/mL) and ionomycin (400 ng/mL) for 48 hours, then stained with Il2ra monoclonal antibody, clone 7D4 (Cat # MAB3228)-FITC and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Il2ra
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Publication Reference
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IL-2 receptor alpha chain (CD25, TAC) expression defines a crucial stage in pre-B cell development.
Rolink A, Grawunder U, Winkler TH, Karasuyama H, Melchers F.
International Immunology 1994 Aug; 6(8):1257.
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IL-2 receptor alpha chain expression during early B lymphocyte differentiation.
Chen J, Ma A, Young F, Alt FW.
International Immunology 1994 Aug; 6(8):1265.
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Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site.
Moreau JL, Nabholz M, Diamantstein T, Malek T, Shevach E, Theze J.
European Journal of Immunology 1987 Jul; 17(7):929.
Application：Func, IA, Mouse, C30-1 cells.
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IL-2 receptor alpha chain (CD25, TAC) expression defines a crucial stage in pre-B cell development.
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