Il2ra recombinant monoclonal antibody, clone 2E4
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More Files
- More Functions
Western Blot
Western blot analysis of mouse lymph node tissue lysates with Il2ra recombinant monoclonal antibody, clone 2E4 (Cat # RAB03200).
Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Il2ra recombinant monoclonal antibody, clone 2E4 (Cat # RAB03200).
Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ cover-slips stained with the chimeric r version of RAB03200 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03200- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of mouse splenocytes with Il2ra recombinant monoclonal antibody, clone 2E4 (Cat # RAB03200).
Mouse splenocytes were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of RAB03200 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Il2ra.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to mouse Il2ra.
Reactivity
Mouse
Form
Liquid
Isotype
IgG
Recommend Usage
Flow Cytometry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of mouse lymph node tissue lysates with Il2ra recombinant monoclonal antibody, clone 2E4 (Cat # RAB03200).Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Il2ra recombinant monoclonal antibody, clone 2E4 (Cat # RAB03200).
Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ cover-slips stained with the chimeric r version of RAB03200 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03200- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.Flow Cytometry
Flow cytometric analysis of mouse splenocytes with Il2ra recombinant monoclonal antibody, clone 2E4 (Cat # RAB03200).
Mouse splenocytes were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of RAB03200 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — Il2ra
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