Il2ra recombinant monoclonal antibody, clone PC-61.5.3
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More Files
- More Functions
Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Il2ra recombinant monoclonal antibody, clone PC-61.5.3 (Cat # RAB03201).
Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric r version of RAB03201 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03201- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Il2ra.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against the B6.1 mouse cytotoxic T cell line, followed by fusing spleen cells with P3X63Ag8.653 myeloma cells.
Reactivity
Mouse
Form
Liquid
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Functional Study
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Il2ra recombinant monoclonal antibody, clone PC-61.5.3 (Cat # RAB03201).
Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric r version of RAB03201 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03201- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.Enzyme-linked Immunoabsorbent Assay
Functional Study
Flow Cytometry
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Gene Info — Il2ra
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