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Last updated: 2021/6/6

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S1PR1 monoclonal antibody, clone 8E4H5 

  • Catalog # : MAB21370
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant human S1PR1.
  • Immunogen:
  • Recombinant protein corresponding to human S1PR1.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 42.8
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • ELISA (1:10000)
    Flow Cytometry (1:200-1:400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of Lane 1: HEK293 and Lane 2: S1PR1-hIgGFc transfected HEK293 cell lysates with S1PR1 monoclonal antibody, clone 8E4H5 (Cat # MAB21370).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human ovarian cancer with S1PR1 monoclonal antibody, clone 8E4H5 (Cat # MAB21370).
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA analysis with S1PR1 monoclonal antibody, clone 8E4H5 (Cat # MAB21370).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HL-60 cells with S1PR1 monoclonal antibody, clone 8E4H5 (Cat # MAB21370) (Green). Red: Negative Control.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1901
  • Gene Name:
  • S1PR1
  • Gene Alias:
  • CHEDG1,D1S3362,ECGF1,EDG-1,EDG1,FLJ58121,S1P1
  • Gene Description:
  • sphingosine-1-phosphate receptor 1
  • Gene Summary:
  • The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. [provided by RefSeq
  • Other Designations:
  • G protein-coupled sphingolipid receptor,OTTHUMP00000012525,endothelial differentiation, sphingolipid G-protein-coupled receptor, 1,sphingosine 1-phosphate receptor EDG1
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