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Last updated: 2018/1/21
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MAD2L1 monoclonal antibody, clone 17D10

  • Catalog # : MAB1926
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant.
  • Immunogen:
  • Recombinant protein corresponding to full length human MAD2L1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • Western Blot (1:200-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using MAD2L1 monoclonal antibody, clone 17D10 (Cat # MAB1926) shows detection of aband at ~24 KDa (arrowhead) correspondingto MAD2L1 present in a HeLa whole cell lysate (Lane 1).
    Approximately 75 ug of lysate was separated by 4-20% TG SDS-PAGE.
    After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:200.
    The membrane was washed and reacted with a 1 : 5,000 dilution of IRDye™800 conjugated Sh-a-Mouse IgG [H&L] for 45 minat room temperature (800 nm channel, green).
    Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
    IRDye™800 fluorescence image was captured using the Odyssey® InfraredImaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc. Otherdetection systems will yield similar results.
  • Immunoprecipitation
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 4085
  • Protein Accession#:
  • Q13257;NP_002349
  • Gene Name:
  • MAD2L1
  • Gene Alias:
  • Gene Description:
  • MAD2 mitotic arrest deficient-like 1 (yeast)
  • Gene Summary:
  • MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq
  • Other Designations:
  • MAD2 (mitotic arrest deficient, yeast, homolog)-like 1,MAD2-like 1,MAD2-like protein 1,mitotic arrest deficient, yeast, homolog-like 1
  • Gene Pathway
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