ACLY monoclonal antibody, clone 5F8D11
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) HeLa cell, (2) NIH/3T3 cell, (3) C6 cell, (4) COS7 cell, (5) Raji cell with ACLY monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissue with ACLY monoclonal antibody.
Immunocytochemistry
Immunocytochemical staining of HeLa cells with ACLY monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ACLY monoclonal antibody, clone 5F8D11.
Flow Cytometry
Flow cytometric analysis of HeLa cells with ACLY monoclonal antibody (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human ACLY.
Immunogen
Recombinant protein corresponding to amino acids 306-502 of human ACLY from E. coli.
Host
Mouse
Theoretical MW (kDa)
125
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:50)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) HeLa cell, (2) NIH/3T3 cell, (3) C6 cell, (4) COS7 cell, (5) Raji cell with ACLY monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded esophageal cancer tissue with ACLY monoclonal antibody.Immunocytochemistry
Immunocytochemical staining of HeLa cells with ACLY monoclonal antibody (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments labeled with Alexa Fluor-555 phalloidin (red).Enzyme-linked Immunoabsorbent Assay
ELISA analysis of ACLY monoclonal antibody, clone 5F8D11.Flow Cytometry
Flow cytometric analysis of HeLa cells with ACLY monoclonal antibody (green) and negative control (purple). -
Gene Info — ACLY
Entrez GeneID
47Gene Name
ACLY
Gene Alias
ACL, ATPCL, CLATP
Gene Description
ATP citrate lyase
Omim ID
108728Gene Ontology
HyperlinkGene Summary
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000164773
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Metabolic pathways
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