ACLY recombinant monoclonal antibody, clone 3A5
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: L02 whole cell lysate, Lane 2: K562 whole cell lysate, Lane 3: Hela whole cell lysate, Lane 4: Raji whole cell lysate and Lane 5: A549 whole cell lysate with ACLY recombinant monoclonal antibody, clone 3A5 (Cat # RAB04398).
Immunofluorescence
Immunofluorescent staining of HepG2 cells with ACLY recombinant monoclonal antibody, clone 3A5 (Cat # RAB04398) (diluated at 1:50). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Flow Cytometry
Flow cytometric analysis of HeLa cells with ACLY recombinant monoclonal antibody, clone 3A5 (Cat # RAB04398) (diluated at 1:50; purple line) and negative control (green line).
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ACLY.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ACLY.
Theoretical MW (kDa)
Calculated MW: 121,
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry (1:20-1:200)
Immunofluorescence (1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Western blot analysis of Lane 1: L02 whole cell lysate, Lane 2: K562 whole cell lysate, Lane 3: Hela whole cell lysate, Lane 4: Raji whole cell lysate and Lane 5: A549 whole cell lysate with ACLY recombinant monoclonal antibody, clone 3A5 (Cat # RAB04398).Immunofluorescence
Immunofluorescent staining of HepG2 cells with ACLY recombinant monoclonal antibody, clone 3A5 (Cat # RAB04398) (diluated at 1:50). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells with ACLY recombinant monoclonal antibody, clone 3A5 (Cat # RAB04398) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — ACLY
Entrez GeneID
47Protein Accession#
P53396Gene Name
ACLY
Gene Alias
ACL, ATPCL, CLATP
Gene Description
ATP citrate lyase
Omim ID
108728Gene Ontology
HyperlinkGene Summary
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000164773
-
Interactome
-
Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Metabolic pathways
+ View More Disease
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com