ACLY recombinant monoclonal antibody, clone R02-9B5
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More Files
- More Functions
Western Blot
Western blot analysis of ATP citrate lyase in K562, C6, 3T3, Hela lysates using human ATP citrate lyase recombinant monoclonal antibody, clone R02-9B5 (Cat # RAB01579).
Immunocytochemistry
Immunocytochemistry analysis of ATP Citrate lyase (green) in A549 using human ATP citrate lyase recombinant monoclonal antibody, clone R02-9B5 (Cat # RAB01579), and DAPI(blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human ATP citrate lyase.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human ATP citrate lyase
Theoretical MW (kDa)
Calculated MW: 121 k
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of ATP citrate lyase in K562, C6, 3T3, Hela lysates using human ATP citrate lyase recombinant monoclonal antibody, clone R02-9B5 (Cat # RAB01579).Immunocytochemistry
Immunocytochemistry analysis of ATP Citrate lyase (green) in A549 using human ATP citrate lyase recombinant monoclonal antibody, clone R02-9B5 (Cat # RAB01579), and DAPI(blue).Immunoprecipitation
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Gene Info — ACLY
Entrez GeneID
47Protein Accession#
P53396Gene Name
ACLY
Gene Alias
ACL, ATPCL, CLATP
Gene Description
ATP citrate lyase
Omim ID
108728Gene Ontology
HyperlinkGene Summary
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000164773
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Metabolic pathways
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