ATP-Citrate Lyase recombinant monoclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts of Lane1:Jurkat cell, Lane2:HeLa cell, Lane3:Rat lung cell, Lane4:Rat liver cell with ATP-Citrate Lyase recombinant monoclonal antibody (Cat # RAB02401).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of paraffin-embedded human breast cancer using ATP-Citrate Lyase recombinant monoclonal antibody (Cat # RAB02401) at dilution of 1:150 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against recombinant ATP-Citrate Lyase.
Antibody Species
Rabbit
Theoretical MW (kDa)
120
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of ATP-Citrate Lyase and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry(1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)Storage Buffer
In PBS, pH 7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of extracts of Lane1:Jurkat cell, Lane2:HeLa cell, Lane3:Rat lung cell, Lane4:Rat liver cell with ATP-Citrate Lyase recombinant monoclonal antibody (Cat # RAB02401).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of paraffin-embedded human breast cancer using ATP-Citrate Lyase recombinant monoclonal antibody (Cat # RAB02401) at dilution of 1:150 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.Immunofluorescence
Flow Cytometry
-
Gene Info — ACLY
Entrez GeneID
47Protein Accession#
P53396Gene Name
ACLY
Gene Alias
ACL, ATPCL, CLATP
Gene Description
ATP citrate lyase
Omim ID
108728Gene Ontology
HyperlinkGene Summary
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000164773
-
Interactome
-
Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Metabolic pathways
+ View More Disease
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com