BCL2 monoclonal antibody, clone P22-A
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More Files
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human BCL2.
Immunogen
A synthetic peptide corresponding to amino acids 42-60 at N-terminus of human BCL2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
EVAC purification
Isotype
IgG
Recommend Usage
ELISA (1:50000-1:100000)
Immunocytochemistry
Immunoprecipitation
Western Blot (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl buffer, pH 8.0 (10 mg/mL BSA, 0.05% Sodium Azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: HEK293 cell lysate 100 ug, Lane 2: HEK293 cell lysate 200 ug, Lane 3: BCL2 recombinant protein 100 ng and Lane 4: BCL2 recombinant protein 200 ng with BCL2 monoclonal antibody, clone P22-A (Cat # MAB15916).Immunocytochemistry
Immunocytochemical staining of HEK293 cells with BCL2 monoclonal antibody, clone P22-A (Cat # MAB15916) at 1:200 dilution.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — BCL2
Entrez GeneID
596Protein Accession#
P10415Gene Name
BCL2
Gene Alias
Bcl-2
Gene Description
B-cell CLL/lymphoma 2
Omim ID
151430Gene Ontology
HyperlinkGene Summary
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq
Other Designations
B-cell lymphoma protein 2|OTTHUMP00000163680
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Interactome
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Pathway
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Disease
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Publication Reference
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Stereotactic Administration of Edaravone Ameliorates Collagenase-Induced Intracerebral Hemorrhage in Rat.
Zhang Y, Yang Y, Zhang GZ, Gao M, Ge GZ, Wang QQ, Ji XC, Sun YL, Zhang HT, Xu RX.
CNS Neuroscience & Therapeutics 2016 Oct; 22(10):824.
Application:IF, IHC, Rat, Rat brain.
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Recombinant adeno-associated virus-delivered anginex inhibits angiogenesis and growth of HUVECs by regulating the Akt, JNK and NF-κB signaling pathways.
Ma K, Wang C, Geng Q, Fan Y, Ning J, Yang H, Dong X, Dong D, Guo Y, Wei X, Li E, Wu Y.
Oncology Reports 2016 Jun; 35(6):3505.
Application:WB-Ce, Human, HUVECs.
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Stereotactic Administration of Edaravone Ameliorates Collagenase-Induced Intracerebral Hemorrhage in Rat.
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