BCL2 recombinant monoclonal antibody, clone 2C6
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human BCL2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human BCL2.
Theoretical MW (kDa)
Calculated MW: 26 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunohistochemistry (1:50-1:500)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: HepG2 whole cell lysate, Lane 2: Jurkat whole cell lysate and Lane 3: MCF-7 whole cell lysate with BCL2 recombinant monoclonal antibody, clone 2C6 (Cat # RAB04217).Immunohistochemistry
Immunohistochemical staining of human thyroid tissue with BCL2 recombinant monoclonal antibody, clone 2C6 (Cat # RAB04217) (diluated at 1:100).Immunohistochemistry
Immunohistochemical staining of human lymph node tissue with BCL2 recombinant monoclonal antibody, clone 2C6 (Cat # RAB04217) (diluated at 1:100).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Jurkat cells with BCL2 recombinant monoclonal antibody, clone 2C6 (Cat # RAB04217) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — BCL2
Entrez GeneID
596Protein Accession#
P10415Gene Name
BCL2
Gene Alias
Bcl-2
Gene Description
B-cell CLL/lymphoma 2
Omim ID
151430Gene Ontology
HyperlinkGene Summary
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq
Other Designations
B-cell lymphoma protein 2|OTTHUMP00000163680
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Interactome
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