Rabbit monoclonal antibody raised against synthetic trimethyl peptide of human Histone H4 (trimethyl K20).
A synthetic trimethyl peptide corresponding to residues surrounding K20 of Histone H4.
This antibody reacts to Histone H4 trimethylated at Lysine 20. It may slightly cross react to dimethylated Lysine 20 at a higher concentration. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20, or other methylations in histone H4.
Protein A purification
ELISA (0.5 ug/mL-1 ug/mL) Western Blot (1 ug/mL-2 ug/mL) The optimal working dilution should be determined by the end user.
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: acid extracts of HeLa cell and Lane 2: recombinant Histone H4 with Histone H4 (trimethyl K20) monoclonal antibody, clone RM208 (Cat # MAB12799) at 1 ug/mL working concentration, showed a band of Histone H4 trimethylated at Lysine 20.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq