H4C1 recombinant monoclonal antibody, clone R05-8H9
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More Files
- More Functions
Western Blot
Western Blot analysis of Hela lysates with H4C1 recombinant monoclonal antibody, clone R05-8H9 (Cat # RAB02219).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with H4C1 recombinant monoclonal antibody, clone R05-8H9 (Cat # RAB02219). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human H4C1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human H4C1.
Theoretical MW (kDa)
Calculated MW: 11 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
ChIP(1:20)
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot
Western Blot analysis of Hela lysates with H4C1 recombinant monoclonal antibody, clone R05-8H9 (Cat # RAB02219).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with H4C1 recombinant monoclonal antibody, clone R05-8H9 (Cat # RAB02219). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry
Immunofluorescence
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Gene Info — HIST4H4
Entrez GeneID
121504Protein Accession#
P62805Gene Name
HIST4H4
Gene Alias
H4/p, MGC24116
Gene Description
histone cluster 4, H4
Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq
Other Designations
histone 4, H4|histone H4
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Interactome
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Pathway
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