Histone H4 (acetyl K5) recombinant monoclonal antibody, clone R08-7D2
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human histone H4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic acetyl peptide corresponding to residues surrounding K5 of human histone H4.
Theoretical MW (kDa)
Calculated MW: 11 kD
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of 3T3 whole cell lysate with Histone H4C (acetyl K5) recombinant monoclonal antibody, clone R08-7D2 (Cat # RAB04411).Immunohistochemistry
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with Histone H4 (acetyl K5) recombinant monoclonal antibody, clone R08-7D2 (Cat # RAB04411) (green). DAPI was stained the cell nucleus (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — HIST4H4
Entrez GeneID
121504Protein Accession#
P62805Gene Name
HIST4H4
Gene Alias
H4/p, MGC24116
Gene Description
histone cluster 4, H4
Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq
Other Designations
histone 4, H4|histone H4
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Interactome
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Pathway
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