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Last updated: 2017/9/24

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ACLY monoclonal antibody

  • Catalog # : MAB11167
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant protein of ACLY.
  • Immunogen:
  • Recombinant protein corresponding to C-terminus of human ACLY.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:5000-1:20000)
    Western Blot (1:100-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (1% BSA, 50% glycerol, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of ACLY monoclonal antibody (Cat # MAB11167) at 1:1000 dilution.
    Lane 1: K-562 whole cell lysate 40 ug/lane.
    Lane 2: Jurkat whole cell lysate 40 ug/lane.
    Lane 3: HeLa whole cell lysate 40 ug/lane.
    Predicted band size: 120 KDa. Observed band size: 120 KDa
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 47
  • Gene Name:
  • ACLY
  • Gene Alias:
  • ACL,ATPCL,CLATP
  • Gene Description:
  • ATP citrate lyase
  • Gene Summary:
  • ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000164773
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