PTK2 monoclonal antibody, clone 10H7E9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using PTK2 monoclonal antibody, clone 10H7E9 (Cat # MAB10841) against PTK2-hIgGFc transfected HEK293 cell lysate.
Immunofluorescence
Immunofluorescence analysis of A-549 cells using PTK2 monoclonal antibody, clone 10H7E9 (Cat # MAB10841) (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of Raji cells using PTK2 monoclonal antibody, clone 10H7E9 (Cat # MAB10841) (green) and negative control (purple).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant PTK2.
Immunogen
Recombinant protein corresponding to human PTK2.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using PTK2 monoclonal antibody, clone 10H7E9 (Cat # MAB10841) against PTK2-hIgGFc transfected HEK293 cell lysate.Immunofluorescence
Immunofluorescence analysis of A-549 cells using PTK2 monoclonal antibody, clone 10H7E9 (Cat # MAB10841) (green). Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Raji cells using PTK2 monoclonal antibody, clone 10H7E9 (Cat # MAB10841) (green) and negative control (purple). -
Gene Info — PTK2
Entrez GeneID
5747Gene Name
PTK2
Gene Alias
FADK, FAK, FAK1, pp125FAK
Gene Description
PTK2 protein tyrosine kinase 2
Omim ID
600758Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. [provided by RefSeq
Other Designations
focal adhesion kinase 1
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Interactome
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Pathway
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Disease
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