PTK2 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane1:Hela whole cell lysate Lane2:The spleen tissue lysate of Mouse with PTK2 recombinant monoclonal antibody (Cat # RAB02694) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using PTK2 recombinant monoclonal antibody (Cat # RAB02694). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of PANC-1 cells using PTK2 recombinant monoclonal antibody (Cat # RAB02694)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against PTK2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant PTK2.
Theoretical MW (kDa)
125
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of FAK and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane1:Hela whole cell lysate Lane2:The spleen tissue lysate of Mouse with PTK2 recombinant monoclonal antibody (Cat # RAB02694) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using PTK2 recombinant monoclonal antibody (Cat # RAB02694). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of PANC-1 cells using PTK2 recombinant monoclonal antibody (Cat # RAB02694)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
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Gene Info — PTK2
Entrez GeneID
5747Protein Accession#
Q05397Gene Name
PTK2
Gene Alias
FADK, FAK, FAK1, pp125FAK
Gene Description
PTK2 protein tyrosine kinase 2
Omim ID
600758Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. [provided by RefSeq
Other Designations
focal adhesion kinase 1
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Interactome
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Pathway
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