PTK2 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates with Focal Adhesion Kinase recombinant monoclonal antibody (Cat # RAB02495).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human colon cancer formalin fixed paraffin embedded tissue section using Focal Adhesion Kinase recombinant monoclonal antibody (Cat # RAB02495). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.56). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescence
Immunofluorescent analysis of A549 cells with Focal Adhesion Kinase recombinant monoclonal antibody (Cat # RAB02495). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PTK2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human FAK.
Theoretical MW (kDa)
125
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of Focal Adhesion Kinase protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:10-1:50)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates with Focal Adhesion Kinase recombinant monoclonal antibody (Cat # RAB02495).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human colon cancer formalin fixed paraffin embedded tissue section using Focal Adhesion Kinase recombinant monoclonal antibody (Cat # RAB02495). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.56). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of A549 cells with Focal Adhesion Kinase recombinant monoclonal antibody (Cat # RAB02495). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.Immunoprecipitation
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Gene Info — PTK2
Entrez GeneID
5747Protein Accession#
Q05397Gene Name
PTK2
Gene Alias
FADK, FAK, FAK1, pp125FAK
Gene Description
PTK2 protein tyrosine kinase 2
Omim ID
600758Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. [provided by RefSeq
Other Designations
focal adhesion kinase 1
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Interactome
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Pathway
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Disease
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